Thin layer chromotography of analgesic drugs introduction chromatographic are used extensively in organic chemistry laboratories for routines analysis. Pdf different spectrophotometric and tlcdensitometric. Introduction chromatographic are used extensively in organic chemistry laboratories for routines analysis. Chromatography in the detection and characterization of. Thin layer chromatography tlc analysis of analgesic drugs. Pain and discomfort in everyday life are often treated with overthecounter otc analgesic medications. Oct 12, 2011 recognize opioid receptor mixed agonistantagonists and pure antagonists. The purpose of this experiment is to use thinlayer chromatography tlc to determine the composition of the analgesic. January 24, 20 tlc analysis of analgesic drugs introduction. Separation and analysis of ingredients in analgesic tablets. The possible unknowns and their analgesic ingredients will be anacin aspirin, caffeine, excedrin acetaminophen. Essay lab report on tlc analisys of analgestic drugs.
Analysis of analgesic, antipyretic, and nonsteroidal anti. In this experiment the tlc was used to examine the composition of known analgesic whic is the pain relieving drug such as paracetamol. Classify nonsteroidal antiinflammatory drugs nsaids. Chem 344 thin layer chromatography thin layer chromatography tlc is a useful technique for the separation and identification of compounds in mixtures. Pdf chromatography analyzing analgesics by tlc and. Pdf thin layer chromotography of analgesic drugs idayu. The purpose of this experiment is to use thin layer chromatography to identify analgesic in a mixture, to identify the unknown analgesic by comparing the r f value, to find and determine the r f for each analgesic. All of them should be written in paragraphs and the method should not. Nonnarcotic or nonopioid nsaid,s and aspirin like analgesics. Other hplc experiments that involve analgesic mixtures have appeared in this journal, 14, but these do not incorporate quantitative analyses.
Analgesics are drugs that relieve pain due to multiple causes. The skills by the end of the experiment you should be able to. Have you taken the safety quiz on the chemsafety quiz sakai site. Tlc analysis of analgesics chem 21l unc charlotte studocu. The purpose of this lab was to determine the composition of four different overthecounter drugs anacin, bufferin, excedrin, and tylenol. Overthecounter otc analgesics typically contain one or more of the following active ingredients. Different spectrophotometric and tlcdensitometric methods for determination of two analgesic drugs article pdf available april 2014 with 56 reads how we measure reads. Separation of the components of an analgesic tablet ii. E29 preparation of aspirin acetylsalicylic acid and thin. From the array of components you discover by tlc, you will be able to identify the otc drug name from table 1. Up to 70% of the population in western countries uses analgesics regularly, primarily for. Immerse the pointed end of a capillary tube into the test tube containing the sample. Quantitative hplc analysis of an analgesiccaffeine. Experiment 12 tlc analysis of analgesic drugs free download as word doc.
Tlc analysis, or thinlayer chromatography, refers to a laboratory technique used heavily in organic chemistry experiments to identify the specific components of a substance by determining the retention factor rf of the particular compound being tested and comparing it to the known retention factor values of other compounds. If you use any reference, please, write the complete. Tlc is used routinely to follow the progress of reactions by monitoring the consumption of starting materials and the appearance of products. Start studying quiz 4 thin layer chromatography of analgesics. Thin layer chromatography tlc is a solidliquid technique in which the two phases are a solid stationary phase and a liquid moving phase. The most common pain relief drugs include aspirin, paracetamol, and.
Pdf in organic chemistry, chromatography refers to the separation of a mixture or ions based on their molecular interactions with two separate. The powdered side of the silica gel plate, not the shiny, plastic side is the side that is spotted. Introduction the purpose of this lab is to use thinlayer chromatography to determine the composition of a series of painkillers. Whether it is a headache or muscular pain, three of the major pain relievers are aspirin, acetaminophen, and ibuprofen. Thin layer chromatography is a useful means of quickly characterizing the main active ingredients of certain commercial analgesics containing aspirin, caffeine, acetaminophen, and ibuprofen when they do not have many other organic compounds in significant proportions. Learning objectives understand the basic principles of polarity of organic compounds. A modern high performance liquid chromatography hplc laboratory experiment which entails the separation of acetaminophen, aspirin, and caffeine and the quantitative assay of caffeine in commercial mixtures of these compounds has been developed. In this experiment, thin layer chromatography tlc will be used to identify the active ingredients in some of these products. Thin layer chromotography of analgesic drugs objectives 1 determine the rf value. Separation and analysis of ingredients in analgesic. In this experiment, a thinlayer chromatography tlc was used to determine the composition of various overthecounter analgesics. Always wear appropriate gloves, long sleeve lab coats, and full.
Thinlayer chromatography mohrig, 2nd edition, technique 15, pp. You will need to be familiar with some terms prior to the lab. Simultaneous densitometric determination of aspirin and omeprazole by highperformanee thinlayer chromatography. In this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. Thinlayer chromatography of analgesics introduction in this experiment, you will use thin. As the reaction proceeds, the solid salicylic acid disappears and the acetylsalicylic acid product.
After completing this experiment, the student should be able to. Students extract, isolate, and perform thin layer chromatography on aspirin, acetominophen, or ibuprofen. May 03, 20 antipyretic, antiinflammatory and analgesic drugs. Analysis of analgesic tablets by highperformance liquid chromatography1 nonprescription headache medications such as excedrin or vanquish contain mixtures of acetaminophen and aspirin for relief and caffeine as a stimulant. This ratio is always constant, regardless how long the tlc plate is. Both of these adsorbents are polar, but alumina is more so. Tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics. The purpose of this experiment is to identify an unknown proprietary drug using thinlayer chromatography. Tlc analysis of ingredients in analgesic agents on hptlc silica gel 60 rp18 wf 254s with concentrating zone analyte set 3.
Essay about tlc analysis of analgesic drugs 625 words cram. Uv lamp and iodine are used to visualized the components. Tlc can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. Terms in this set 29 what is the purpose of tlc of analgesic drugs. This paper describes a modern experiment for the quan.
In addition to control of the dietary intake of fat, particularly saturated fatty acids, antihyperlipidemic drug. Chem 2 fall 2008 identification of over thecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the progress of a reaction. Perform analysis on seized illicit and pharmaceutical drugs and related materials. Clearly, an updated quantitative experiment for this drug combination is warranted. Experimental procedure, report template introduction in this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. Experimental procedure, report template introduction in this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the samples of aspirin. The pump draws eluent from a reservoir and delivers it to the column.
In this lab, you will perform thin layer chromatography tlc to identify an unknown drug and then use column chromatography to separate out the components of. These drugs are remarkably safe, but serious side effects can occur. Be able to deduce the relative polarity of organic compounds by comparing the functional groups. Tlc key thinlayer chromatography lab complete answer key. Several common analgesics are aspirin and acetomeniphen. The experiment is appropriate for any chemistry student who has completed a minimum of one year of general chemistry and is ideal for an analytical or instrumental analysis course. In forensic analysis, thin layer chromatography tlc is also a classic, simple, and versatile method for drug analysis. List the therapeutic uses of nsaids, side effects, contraindications and toxicity. Be sure to include all the analgesics and solvents used in this experiment in your chemical data tables. Analgesic activity of the methanolic seed extract of buchholzia coriacea. South encyclopedia of life support systems eolss 8. The purpose of this lab was to determine the composition of four different overthecounter drugs anacin, bufferin, excedrin, and tylenol these compositions were determined by using the method of thinlayer chromatography tlc of the four overthecounter drugs, which were then compared to four.
Salicylic acid is the substance that has analgesic, antiinflammatory, and antipyretic properties. Thinlayer chromatography tlc is a technique with the advantages that it is easy to implement and cheap. Thin layer chromatography rev 22017 thin layer chromatography tlc is a method for analyzing mixtures by separating the compounds in the mixture based on polarity. You will be given five known reference substances, an unknown.
The unknowns behavior in thinlayer chromatography will be compared with that of its possible component analgesics. Separation of the components of an analgesic tablet i. Tlc analysis of ingredients in analgesic agents on hptlc. Precut plastic backed or metal backed tlc plates, tlc capillaries, uv lamp aim the goal of your experiment is two fold. Solved experiment 3 tlc analysis of analgesic drugs. Tlc is limited, however, in terms of secure characterization and is therefore often used in parallel with other methods of structural analysis. Thin layer chromatography analysis of analgesic drugs essay. Jce0498 p0467 quantitative hplc analysis of an analgesic. Essay about tlc analysis of analgesic drugs 7 words cram. A few common analgesic drugs will be analyzed and an unknown analgesic drug will be identified via the thin layer chromatography technique. Ideal for the first week of an organic chemistry sequence to get students familiar with their surroundings. Tlc is a type of adsorption chromatography, and the most common substrates used for the stationary phases in the lab, are silica sio 2 and alumina al 2 o 3.
Whether it is a headache or muscular pain, three of the major. The analgesics that could be in the tablets included ibuprofen, acetaminophen, aspirin, a. Thin layer chromatographic profiling and evaluation of. Please turn in the answers to these questions when you arrive to your first lab.
Lab report on tlc analisys of analgestic drugs essay examples. If tlc does not result in bringing blood lipids to therapeutic levels, the primary health care provider may add one of the antihyperlipidemic drugs to the treatment plan. Elizabeth ping october 11, 2011 1 tlc analysis of an analgesic introduction. The principle of using tlc in counterfeit analysis is quite simple. Tlc can be used to help determine the number of components in a. Analysis of drugs by thin layer chromatography experimental techniques required chromatography thinlayer t other documents.
To determine the identities of the analgesic components present in an unknown mixture by comparison with known analgesic samples using tlc. Does anyone got a lab report in tlc analysisi of analgesic drugs so i could understand it better. Experiment 12 tlc analysis of analgesic drugs thin layer. Feimeng zhou chem 463 microanalytical methods spring 2006 objectives. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
An introductory laboratory on the identification of analgesics in an unknown sample. The report should be up to 800 words and it should include. Overthecounter otc analgesics typically contain one or more of the following active. Hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Pdf analysis of analgesic, antipyretic, and nonsteroidal. Our hplc protocol resolves these compounds in only three minutes with a straightforward chromatographic apparatus which consists of a c18 column, an. Acetaminophen, acetylsalicylic acid, caffeine, and ibuprofen. Thinlayer chromatography you will need to bring your package of tlc plates on this day. Identification of analgesic drugs tlc cerritos college.
The objective of this experiment was to analyze analgesic tablets by tlc to determine what analgesics they contain. Spot one plate with your 4 known standards acetaminophen, aspirin. Study guide for drug analysis examination drug analysis study guideversion 1. Design of a drug discovery course for nonscience majors. Tlc analysis of analgesic drugs flashcards quizlet. The purpose of this experiment is to use thinlayer chromatography tlc to determine the composition of the analgesic, pamprin. The purpose of this experiment is to identify the brand name of an unknown commercial analgesic product by tlc analysis.
Perform qualitative analyses of evidential materials using established chemical. Background thin layer chromatography tlc is used routinely in the laboratory to both. All of them should be written in paragraphs and the method should not be copied exactely from the instruction. In such situations, a rational multimodal approach can also result in the fewest adverse effects. Thin layer chromatography, or tlc, is a method for analyzing mixtures by separating the compounds in the mixture. Thin layer chromatography analysis of analgesic drugs essay sample. Analysis of cocaine and crack cocaine via thin layer. Thin layer chromatography tlc a method chemists use to separate one chemical from. To assess the purity of the colored organic compounds provided to you, using tlc. Lab report on tlc analisys of analgestic drugs essay examples 1266 words 6 pages tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics.
Analyzing analgesics by thin layer chromatography tlc. Jpc journal of planar chromatography modern tlc 2019, 32 6, 501504. Thin layer chromatography characterization of the active. Thinlayer chromatography analysis of analgesics adam cap. Draw a light pencil line about 1 cm from the end of each chromatographic plate. May 11, 2014 hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Tlc analysis report of analgesics on hptlc silica gel 60 rp. Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the progress of a reaction. This experiment describes conditions for separating and measuring the components by highperformance liquid. Thin layer chromatography tlc is used routinely in the laboratory to both monitor reactions and analyse the purity of samples. Tlc key thinlayer chromatography lab complete answer. Read all of the required reading at the beginning of the experiment, especially analgesics p.
Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the. Thin layer chromatography tlc analysis of analgesic drugs reading experiment 12 in pavia 5th edition pp 9195. Analgesic and antipyretic activity of methanolic extract of coccinia grandis l. Your drug may contain from one to three of these substances. The powdered side of the silica gel plate, not the shiny, plastic side is the side that is. Separation and analysis of ingredients in analgesic tablets by high performance liquid chromatography hplc by dr. Introduction today on the market, there are a variety of over the counter drugs that can be used to combat the aches and pains encountered in everyday living. We discuss the quantitative analysis of multimodal action using the treatment of pain as a practical example and give examples of its application to some widely used analgesic drugs. Quantitative determination of headache tablets the chromatograph you will be using can best be viewed as a series of components. E29 preparation of aspirin acetylsalicylic acid and thinlayer chromatography of analgesic drugs the task to prepare a pharmaceutical compound and test its purity. Tlc analysis of analgesic drugs aim of the experiment determine the composition of a series of. The purpose of this experiment is to focus on the ingredients that the analgesic drugs may contain. Tlc analysis of analgesics on hptlc silica gel 60 rp18 wf254s analyte set 2 conditions.
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